Biochem/physiol Actions

Cell permeable: no

Reversible: no

Target K_i: 5-10 µM against phosphatidylcholine (PC)-specific phospholipase C

Product does not compete with ATP.

Primary Targetpc-specificphopholipase c

General description

Selective inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC) (Bacillus cereus, K_i = 5-10 µM). Does not inhibit (up to 50 µM) phosphatidylinositol-specific phospholipase C, phospholipase A₂, and phospholipase D. Inhibitor of the sphingomyelinase activity. Also inhibits angiogenesis by preventing the synthesis of basement membrane (BM).

Selective inhibitor of phosphatidylcholine (PC)-specific phospholipase C (K_i = 5-10 µM). Does not inhibit phosphatidylinositol-specific phospholipase C, phospholipase A, or phospholipase D. Also reported to inhibit LPS-stimulated ERK activation via its inhibitory effect on PLC. Inhibits angiogenesis by preventing the synthesis of basement membrane.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Monick, M.M., et al. 1999. J. Immunol. 162, 3005.Lozanski, G., et al. 1997. Biochem. J.328, 271.Maragoudakis, M.E., et al. 1993. Kidney Int. 43, 147.Doppler, C., et al. 1992. AIDS Res. Human Retroviruses8, 245.Schutze, S., et al. 1992. Cell71, 765.Sauer, G., et al. 1990. Cancer Lett.53, 97.Muller-Decker, K. 1989. Biochem. Biophys. Res. Commun.162, 198.Amtmann, E., et al. 1987. Biochem. Pharmacol.36, 1545.Sauer, G., et al. 1984. Proc. Natl. Acad. Sci. USA81, 3263.

Packaging

5 mg in Plastic ampoule

Preparation Note

tissue culture media should be at neutral pH, since product is active only at or slightly below pH 7.0. The compound is inactive at pH 7.4 and above, and it is hydrolyzed below pH 6.0. HEPES buffer should not be used as it renders D609 toxic.

Reconstitution

Material is very labile once in solution (1.5 day half-life in tissue culture medium).

Warning

Toxicity: Standard Handling (A)